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KMID : 0381120170390121387
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Genes and Genomics
2017 Volume.39 No. 12 p.1387 ~ p.1394
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Development of SSR molecular markers for Allium mongolicum
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Hu Jing
Hu Xiaoke
Zhang Jinhu
Fan Baoli
Yu Qiushi
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Abstract
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Allium mongolicum is high palatability, nutrient, medicinal value and drought resistance wild plant. However, a lack of molecular markers of this plant results in a series of genetic questions remain largely unknown, including molecular marker data, population genetic structure and accuracy of genetic breeding. In this study, a total of 1,409,706 quality-filtered and trimmed reads were obtained from the normalized genomic DNA of pooled A. mongolicum individuals. These sequences were assembled into 2,093,593 high quality contigs, and a total of 219 simple sequence repeats loci were screened. Sixty of them were selected to validate amplification and to determine the degree of polymorphism in the genomic DNA pools. Fifteen primer pairs successfully amplified DNA fragments and detected significant amounts of polymorphism. The number of alleles in ten geographically diverse A. mongolicum populations ranged from 3 to 8 per locus. The observed and expected heterozygosities ranged from 0.350 to 0.860 and 0.516 to 0.931, respectively. Our results provide a valuable resource for A. mongolicum research.
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KEYWORD
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Allium mongolicum, Genomic DNA, SSR molecular markers, Genetic diversity, Polymorphism
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